Testing Blood Groups?
Answer:
Hello, glad to hear of you curiosity roughly this topic.
In a clinical blood bank, blood groups are tested by first obtain a sample from a merciful who may be transfused.
A small volume of blood from the patient is wash a few times in saline to remove any antibodies found contained by the patients serum (This is done because the patient may hold auto-antibodies which may confuse results).
The red blood cell are usually in 3 cup test tubes (just give or take a few 200 RBC's are needed per tube).
To each assessment tube is added pure (commercial grade) antibodies.
In one tube will go the anti-A antibodies, surrounded by another will go anti-B antibodies, and surrounded by a third tube will go anti-AB antibodies.
I haven't perform this test surrounded by a while, so maybe I am forgetting a step, but after the antibodies are added, you consent to the tube to sit for a few minutes.
Then you check the tubes for agglutination (clumping of the red blood cells) by visual inspection. I take out using the aid of a mirror and a lamp, to back gauge RBC clumping.
If the tube near the anti-A antibodies show agglutination, then you enjoy a patient that expresses the ABO blood group "A" on the surface of their RBC's. At this point you may own to do what is called a sub-type for "A". Some race are A1 and some are A2, and they are not necessarily compatible.
If the tube with the anti-B antibodies show agglutination, after you have a merciful that expresses the ABO blood group "B" on the surface of their RBC's.
If the tube with the anti-AB antibodies show agglutination, next you have a lenient that expresses the ABO blood group "AB" on the surface of their RBC's.
If no tubes show aggluntiation , then you own a patient that expresses the ABO blood group "O" on the surface of their RBC's.
I'm sure things hold changed since my stint in Blood Banking.
But these are the ground rules.
Hope this helps satiate you curiosity. Pass on the honourable Karma!
They still sell the kit in the chemists. Do a dig out and you'll find them online somewhere - that might tell you what you obligation to know xx
If you can't find a kit - you could other become a blood donor - then you will find out by evasion - and do some good too !
enjoy a look at .
http://blood.co.uk/
We did it in class not to long ago. It took in the order of 1-3 minutes. You need Anti-A, Anti-B, and if you want to know if you are RH positive or unenthusiastic ( what make your blood type a + or a -),you will obligation some Anti-D. Everyone should know their blood type. You only requirement one to two drops of blood..I needed only one (from a finger prick). Mix equal drops of blood to the Anti- you are using. Typically you would hold a tray with three spaces labeled A, B and RH. In the A space you would put one drop blood and one drop Anti-A, if it turns granular, then you do not own type A blood. Do the same point for all three spaces. If the blood looks coarse in the B spot, you do not hold type B blood. If neither type turns grainy afterwards you have O blood. If the Rh turns coarse then you are RH positive. I hope this help.